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faustovirus capping enzyme fce  (New England Biolabs)


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    New England Biolabs faustovirus capping enzyme fce
    Faustovirus Capping Enzyme Fce, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/faustovirus+capping+enzyme+fce/Faustovirus+Capping+Enzyme/pm41389517-111-10-17
    Average 95 stars, based on 32 article reviews
    faustovirus capping enzyme fce - by Bioz Stars, 2026-09
    95/100 stars

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    Purification:

    Article Title: IFIT1 is rapidly evolving and exhibits disparate antiviral activities across 11 mammalian orders
    Article Snippet: RNA was purified according to manufacturer instructions using phenol:chloroform nucleic acid extraction (Promega). .. To generate Cap0 RNA, purified uncapped RNA probes were modified using the Faustovirus Capping Enzyme (FCE) (NEB) using modified manufacturer instructions. .. Briefly, 100μL reactions were composed of 20μL of uncapped RNA purified after T7 transcription (described above), 0.2mM SAM, 1mM GTP, 7500U of FCE, 1x FCE reaction buffer, 80U RNasin® Plus Ribonuclease Inhibitor (Promega), and nuclease-free water up to 100μL.

    Article Title: IFIT1 is rapidly evolving and exhibits disparate antiviral activities across 11 mammalian orders
    Article Snippet: RNA was purified according to the manufacturer instructions using phenol:chloroform nucleic acid extraction (Promega). .. To generate Cap0 RNA, purified uncapped RNA probes were modified using the Faustovirus Capping Enzyme (FCE) (NEB) using modified manufacturer instructions. .. Briefly, 100 μL reactions were composed of 20 μL of uncapped RNA purified after T7 transcription (described above), 0.2 mM SAM, 1 mM GTP, 7500 U of FCE, 1 x FCE reaction buffer, 80 U RNasin Plus Ribonuclease Inhibitor (Promega), and nuclease-free water up to 100 μL.

    Modification:

    Article Title: IFIT1 is rapidly evolving and exhibits disparate antiviral activities across 11 mammalian orders
    Article Snippet: RNA was purified according to manufacturer instructions using phenol:chloroform nucleic acid extraction (Promega). .. To generate Cap0 RNA, purified uncapped RNA probes were modified using the Faustovirus Capping Enzyme (FCE) (NEB) using modified manufacturer instructions. .. Briefly, 100μL reactions were composed of 20μL of uncapped RNA purified after T7 transcription (described above), 0.2mM SAM, 1mM GTP, 7500U of FCE, 1x FCE reaction buffer, 80U RNasin® Plus Ribonuclease Inhibitor (Promega), and nuclease-free water up to 100μL.

    Article Title: IFIT1 is rapidly evolving and exhibits disparate antiviral activities across 11 mammalian orders
    Article Snippet: RNA was purified according to the manufacturer instructions using phenol:chloroform nucleic acid extraction (Promega). .. To generate Cap0 RNA, purified uncapped RNA probes were modified using the Faustovirus Capping Enzyme (FCE) (NEB) using modified manufacturer instructions. .. Briefly, 100 μL reactions were composed of 20 μL of uncapped RNA purified after T7 transcription (described above), 0.2 mM SAM, 1 mM GTP, 7500 U of FCE, 1 x FCE reaction buffer, 80 U RNasin Plus Ribonuclease Inhibitor (Promega), and nuclease-free water up to 100 μL.



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